近日，英国牛津大学Carol V. Robinson小组取得一项新成果。他们的最新研究通过联合天然和“组学”质谱，鉴定出与膜蛋白结合的内源性配体。这一研究成果于2020年5月4日发表在国际学术期刊《自然—方法学》上。

Title: Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

Author: Joseph Gault, Idlir Liko, Michael Landreh, Denis Shutin, Jani Reddy Bolla, Damien Jefferies, Mark Agasid, Hsin-Yung Yen, Marcus J. G. W. Ladds, David P. Lane, Syma Khalid, Christopher Mullen, Philip M. Remes, Romain Huguet, Graeme McAlister, Michael Goodwin, Rosa Viner, John E.P. Syka, Carol V. Robinson

Issue&Volume: 2020-05-04

Abstract: Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, ‘nativeomics’, unifying ‘omics’ (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.

DOI: 10.1038/s41592-020-0821-0

Source: https://www.nature.com/articles/s41592-020-0821-0