美国宾夕法尼亚大学Michael P. Cancro小组在研究中取得进展。他们发现转录因子T-bet区分具有明显组织分布和抗体特异性的记忆B细胞亚群。相关论文于2020年4月29日在线发表在《免疫》杂志上。

Title: The Transcription Factor T-bet Resolves Memory B Cell Subsets with Distinct Tissue Distributions and Antibody Specificities in Mice and Humans

Author: John L. Johnson, Rebecca L. Rosenthal, James J. Knox, Arpita Myles, Martin S. Naradikian, Joanna Madej, Mariya Kostiv, Aaron M. Rosenfeld, Wenzhao Meng, Shannon R. Christensen, Scott E. Hensley, Jonathan Yewdell, David H. Canaday, Jinfang Zhu, Adrian B. McDermott, Yoav Dori, Max Itkin, E. John Wherry, Norbert Pardi, Drew Weissman, Ali Naji, Eline T. Luning Prak, Michael R. Betts, Michael P. Cancro

Issue&Volume: 2020-04-29

Abstract: B cell subsets expressing the transcription factor T-bet are associated with humoralimmune responses and autoimmunity. Here, we examined the anatomic distribution, clonalrelationships, and functional properties of T-bet+ and T-bet memory B cells (MBCs) in the context of the influenza-specific immune response. Inmice, both T-bet and T-bet+ hemagglutinin (HA)-specific B cells arose in germinal centers, acquired memory Bcell markers, and persisted indefinitely. Lineage tracing and IgH repertoire analysesrevealed minimal interconversion between T-bet and T-bet+ MBCs, and parabionts showed differential tissue residency and recirculation properties.T-bet+ MBCs could be subdivided into recirculating T-betlo MBCs and spleen-resident T-bethi MBCs. Human MBCs displayed similar features. Conditional gene deletion studies revealedthat T-bet expression in B cells was required for nearly all HA stalk-specific IgG2cantibodies and for durable neutralizing titers to influenza. Thus, T-bet expressiondistinguishes MBC subsets that have profoundly different homing, residency, and functionalproperties, and mediate distinct aspects of humoral immune memory.

DOI: 10.1016/j.immuni.2020.03.020

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30133-3