美国哈佛医学院Richard I. Sherwood、麻省理工学校David K. Gifford等研究人员合作,利用多重条形码单细胞RNA-Seq方法阐明了驱动ESC分化的组合信号通路。相关论文于2020年5月26日在线发表在《细胞—干细胞》上。
Title: A Multiplexed Barcodelet Single-Cell RNA-Seq Approach Elucidates Combinatorial Signaling Pathways that Drive ESC Differentiation
Author: Grace Hui Ting Yeo, Lin Lin, Celine Yueyue Qi, Minsun Cha, David K. Gifford, Richard I. Sherwood
Issue&Volume: 2020-05-26
Abstract: Empirical optimization of stem cell differentiation protocols is time consuming, islaborintensive, and typically does not comprehensively interrogate all relevant signalingpathways. Here we describe barcodelet single-cell RNA sequencing (barRNA-seq), whichenables systematic exploration of cellular perturbations by tagging individual cellswith RNA “barcodelets” to identify them on the basis of the treatments they receive.We apply barRNA-seq to simultaneously manipulate up to seven developmental pathwaysand study effects on embryonic stem cell (ESC) germ layer specification and mesodermalspecification, uncovering combinatorial effects of signaling pathway activation ongene expression. We further develop a data-driven framework for identifying combinatorialsignaling perturbations that drive cells toward specific fates, including severalannotated in an existing scRNA-seq gastrulation atlas, and use this approach to guideESC differentiation into a notochord-like population. We expect that barRNA-seq willhave broad utility for investigating and understanding how cooperative signaling pathwaysdrive cell fate acquisition.
DOI: 10.1016/j.stem.2020.04.020
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30159-4
Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
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