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中国科学家建立可用于新冠病毒感染测试的转基因小鼠模型
作者：小柯机器人 发布时间：2020/5/22 23:49:37

中科院武汉病毒所石正丽、杨兴娄等研究人员合作建立了表达人类人血管紧张素转化酶2（hACE2）的转基因小鼠，可用于新冠病毒感染测试。相关论文于2020年5月21日在线发表于《细胞》。

据研究人员介绍，自2019年以来，COVID-19已在全球范围内传播，现在已严重威胁公众健康。研究人员之前将该病原鉴定为一种新型的SARS相关冠状病毒（SARS-CoV-2），它使用hACE2作为进入受体。
 
近日，研究人员成功开发了SARS-CoV-2 hACE2转基因小鼠（HFH4-hACE2的C3B6小鼠）感染模型。感染的小鼠产生典型的间质性肺炎和病理，与COVID-19患者相似。病毒定量显示肺是感染的主要部位，尽管在某些小鼠的眼睛、心脏和大脑中也可以发现病毒RNA。从感染的肺和脑组织中分离出全基因组序列中与SARS-CoV-2相同的病毒。最后，研究人员表明预先暴露于SARS-CoV-2可以保护小鼠免受严重的肺炎的侵害。这些结果表明，hACE2小鼠将成为测试潜在疫苗和治疗药物的有力工具。
 
附：英文原文

Title: Pathogenesis of SARS-CoV-2 in transgenic mice expressing human angiotensin-converting enzyme 2

Author: Ren-Di Jiang, Mei-Qin Liu, Ying Chen, Chao Shan, Yi-Wu Zhou, Xu-Rui Shen, Qian Li, Lei Zhang, Yan Zhu, Hao-Rui Si, Qi Wang, Juan Min, Xi Wang, Wei Zhang, Bei Li, Hua-Jun Zhang, Ralph S. Baric, Peng Zhou, Xing-Lou Yang, Zheng-Li Shi

Issue&Volume: 2020-05-21

Abstract: COVID-19 has spread worldwide since 2019 and is now a severe threat to public health. We previously identified the causative agent as a novel SARS-related coronavirus (SARS-CoV-2) that uses human angiotensin-converting enzyme 2 (hACE2) as the entry receptor. Here, we successfully developed a SARS-CoV-2 hACE2 transgenic mouse (HFH4-hACE2 in C3B6 mice) infection model. The infected mice generated typical interstitial pneumonia and pathology that were similar to those of COVID-19 patients. Viral quantification revealed the lungs as the major site of infection, although viral RNA could also be found in the eye, heart, and brain in some mice. Virus identical to SARS-CoV-2 in full-genome sequences was isolated from the infected lung and brain tissues. Lastly, we showed that pre-exposure to SARS-CoV-2 could protect mice from severe pneumonia. Our results show that the hACE2 mouse would be a valuable tool for testing potential vaccines and therapeutics.

DOI: 10.1016/j.cell.2020.05.027

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30622-X

期刊信息
Cell：《细胞》，创刊于1974年。隶属于细胞出版社，最新IF：36.216
官方网址：https://www.cell.com/