俄罗斯科学院Karen S. Sarkisyan研究团队近期取得新进展。他们利用基因工程改造产生了自发光植物。相关论文在线发表在2020年4月27日出版的《自然-生物技术》杂志上。

研究人员设计了一种具有真菌生物发光系统的烟草植物，该系统将咖啡酸（存在于所有植物中）转化为荧光素，并产生了肉眼可见的持续性自发光。该发现为植物成像组件的开发奠定基础。

据介绍，利用质体表达细菌生物发光基因簇产生自发光植物的技术受到光输出低的限制。

附：英文原文

Title: Plants with genetically encoded autoluminescence

Author: Tatiana Mitiouchkina, Alexander S. Mishin, Louisa Gonzalez Somermeyer, Nadezhda M. Markina, Tatiana V. Chepurnyh, Elena B. Guglya, Tatiana A. Karataeva, Kseniia A. Palkina, Ekaterina S. Shakhova, Liliia I. Fakhranurova, Sofia V. Chekova, Aleksandra S. Tsarkova, Yaroslav V. Golubev, Vadim V. Negrebetsky, Sergey A. Dolgushin, Pavel V. Shalaev, Dmitry Shlykov, Olesya A. Melnik, Victoria O. Shipunova, Sergey M. Deyev, Andrey I. Bubyrev, Alexander S. Pushin, Vladimir V. Choob, Sergey V. Dolgov, Fyodor A. Kondrashov, Ilia V. Yampolsky, Karen S. Sarkisyan

Issue&Volume: 2020-04-27

Abstract: Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.

DOI: 10.1038/s41587-020-0500-9

Source: https://www.nature.com/articles/s41587-020-0500-9