德国德累斯顿工业大学Jochen Guck、美国加州大学洛杉矶分校Dino Di Carlo、麻省理工学院Scott R. Manalis等研究人员合作，比较了用于高通量细胞变形能力测量的微流控方法。2020年4月27日，《自然—方法学》在线发表了这一成果。

Title: A comparison of microfluidic methods for high-throughput cell deformability measurements

Author: Marta Urbanska, Hector E. Muoz, Josephine Shaw Bagnall, Oliver Otto, Scott R. Manalis, Dino Di Carlo, Jochen Guck

Issue&Volume: 2020-04-27

Abstract: The mechanical phenotype of a cell is an inherent biophysical marker of its state and function, with many applications in basic and applied biological research. Microfluidics-based methods have enabled single-cell mechanophenotyping at throughputs comparable to those of flow cytometry. Here, we present a standardized cross-laboratory study comparing three microfluidics-based approaches for measuring cell mechanical phenotype: constriction-based deformability cytometry (cDC), shear flow deformability cytometry (sDC) and extensional flow deformability cytometry (xDC). All three methods detect cell deformability changes induced by exposure to altered osmolarity. However, a dose-dependent deformability increase upon latrunculin B-induced actin disassembly was detected only with cDC and sDC, which suggests that when exposing cells to the higher strain rate imposed by xDC, cellular components other than the actin cytoskeleton dominate the response. The direct comparison presented here furthers our understanding of the applicability of the different deformability cytometry methods and provides context for the interpretation of deformability measurements performed using different platforms.

DOI: 10.1038/s41592-020-0818-8

Source: https://www.nature.com/articles/s41592-020-0818-8