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科学家发现人类胸腺定植祖细胞
作者:小柯机器人 发布时间:2020/4/21 12:46:34

近日,比利时根特大学Tom Taghon课题组利用单细胞测序技术,鉴定出出生后人类胸腺定植祖细胞并揭示了未成熟胸腺细胞分化过程中的的调控动态。这一研究成果于2020年4月17日在线发表于《免疫》。

研究人员采用单细胞RNA测序(scRNA-seq)来测试了人类出生后未成熟的胸腺细胞。骨髓和外周血前体数据集的整合确定了两个潜在的胸腺定植祖细胞,它们的CD7、 CD10、归巢受体CCR7、CCR9和ITGB7表达有所不同。
 
尽管两种前体细胞均能够支持T细胞发育,但只有一种可促进胸腺内树突状细胞(DC)分化,主要是浆细胞样树突状细胞。轨迹推断描述了人类T细胞谱系早期发育背后的转录动态,从而能够预测驱动人类T细胞发育中特定阶段的转录因子(TF)集合。
 
这个全面的数据集定义了未成熟人类胸腺细胞的表达特征,并为进一步研究人类胸腺细胞生成提供了资源。
 
据了解,在出生后,胸腺造血依赖于通过血液迁移的骨髓来源造血干细胞在胸腺内的持续定植。目前对胸腺迁移性质的了解主要基于临床前模型的数据。
 
附:英文原文

Title: Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes

Author: Marieke Lavaert, Kai Ling Liang, Niels Vandamme, Jong-Eun Park, Juliette Roels, Monica S. Kowalczyk, Bo Li, Orr Ashenberg, Marcin Tabaka, Danielle Dionne, Timothy L. Tickle, Michal Slyper, Orit Rozenblatt-Rosen, Bart Vandekerckhove, Georges Leclercq, Aviv Regev, Pieter Van Vlierberghe, Martin Guilliams, Sarah A. Teichmann, Yvan Saeys, Tom Taghon

Issue&Volume: 2020-04-17

Abstract: During postnatal life, thymopoiesis depends on the continuous colonization of thethymus by bone-marrow-derived hematopoietic progenitors that migrate through the bloodstream.The current understanding of the nature of thymic immigrants is largely based on datafrom pre-clinical models. Here, we employed single-cell RNA sequencing (scRNA-seq)to examine the immature postnatal thymocyte population in humans. Integration of bonemarrow and peripheral blood precursor datasets identified two putative thymus seedingprogenitors that varied in expression of CD7; CD10; and the homing receptors CCR7,CCR9, and ITGB7. Whereas both precursors supported T cell development, only one contributedto intrathymic dendritic cell (DC) differentiation, predominantly of plasmacytoiddendritic cells. Trajectory inference delineated the transcriptional dynamics underlyingearly human T lineage development, enabling prediction of transcription factor (TF)modules that drive stage-specific steps of human T cell development. This comprehensivedataset defines the expression signature of immature human thymocytes and providesa resource for the further study of human thymopoiesis.

DOI: 10.1016/j.immuni.2020.03.019

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30132-1

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新if:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx