近日,比利时根特大学Tom Taghon课题组利用单细胞测序技术,鉴定出出生后人类胸腺定植祖细胞并揭示了未成熟胸腺细胞分化过程中的的调控动态。这一研究成果于2020年4月17日在线发表于《免疫》。
Title: Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes
Author: Marieke Lavaert, Kai Ling Liang, Niels Vandamme, Jong-Eun Park, Juliette Roels, Monica S. Kowalczyk, Bo Li, Orr Ashenberg, Marcin Tabaka, Danielle Dionne, Timothy L. Tickle, Michal Slyper, Orit Rozenblatt-Rosen, Bart Vandekerckhove, Georges Leclercq, Aviv Regev, Pieter Van Vlierberghe, Martin Guilliams, Sarah A. Teichmann, Yvan Saeys, Tom Taghon
Issue&Volume: 2020-04-17
Abstract: During postnatal life, thymopoiesis depends on the continuous colonization of thethymus by bone-marrow-derived hematopoietic progenitors that migrate through the bloodstream.The current understanding of the nature of thymic immigrants is largely based on datafrom pre-clinical models. Here, we employed single-cell RNA sequencing (scRNA-seq)to examine the immature postnatal thymocyte population in humans. Integration of bonemarrow and peripheral blood precursor datasets identified two putative thymus seedingprogenitors that varied in expression of CD7; CD10; and the homing receptors CCR7,CCR9, and ITGB7. Whereas both precursors supported T cell development, only one contributedto intrathymic dendritic cell (DC) differentiation, predominantly of plasmacytoiddendritic cells. Trajectory inference delineated the transcriptional dynamics underlyingearly human T lineage development, enabling prediction of transcription factor (TF)modules that drive stage-specific steps of human T cell development. This comprehensivedataset defines the expression signature of immature human thymocytes and providesa resource for the further study of human thymopoiesis.
DOI: 10.1016/j.immuni.2020.03.019
Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30132-1
Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新if:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx