近日，美国哈佛大学与麻省理工学院联合的博德研究所John G. Doench等研究人员总结了CRISPR–Cas实验的设计和分析。这一综述论文于2020年4月13日在线发表在国际学术期刊《自然—生物技术》上。
Title: Design and analysis of CRISPR–Cas experiments
Author: Ruth E. Hanna, John G. Doench
Abstract: A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible manipulation of genomes in both targeted and large-scale experiments. Numerous software tools and analytical methods have been developed for the design and analysis of CRISPR–Cas experiments, including resources to design optimal guide RNAs for various modes of manipulation and to analyze the results of such experiments. A major recent focus has been the development of comprehensive tools for use on data from large-scale CRISPR-based genetic screens. As this field continues to progress, a clear ongoing challenge is not only to innovate, but to actively maintain and improve existing tools so that researchers across disciplines can rely on a stable set of excellent computational resources for CRISPR–Cas experiments.