捷克南波西米亚大学Julius Lukeš、Drahomíra Faktorová、美国蒙特雷湾水族馆研究所Alexandra Z. Worden和英国东安格利亚大学Thomas Mock等研究人员合作开发了海洋原生生物的遗传操作工具。这一研究成果于2020年4月6日在线发表在《自然—方法学》杂志上。

研究人员报道了用于分析一系列主要来自海洋环境原生生物的遗传操作工具。研究人员在13个从未实现转化的物种中进行了外来DNA的递送和表达，以及在其他8个物种中遗传工具的开发，并解释了尚未实现对另外17个物种的转化的原因。研究人员在遗传操作方面的资源将提供有关祖先真核生物形式、真核生物细胞生物学、蛋白质多样性和细胞途径进化的认识。

据介绍，多样的微生物生态系统是海洋生物的基础。在这些微生物中，有许多单细胞的真核生物，跨越了真核生物树的多样性。但是，遗传可追踪性仅限于少数几个物种，这些物种无法代表真核生物多样性或与环境有关的分类单元。

附：英文原文

Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Author: Drahomra Faktorov, R. Ellen R. Nisbet, Jos A. Fernndez Robledo, Elena Casacuberta, Lisa Sudek, Andrew E. Allen, Manuel Ares, Cristina Arest, Cecilia Balestreri, Adrian C. Barbrook, Patrick Beardslee, Sara Bender, David S. Booth, Franois-Yves Bouget, Chris Bowler, Susana A. Breglia, Colin Brownlee, Gertraud Burger, Heriberto Cerutti, Rachele Cesaroni, Miguel A. Chiurillo, Thomas Clemente, Duncan B. Coles, Jackie L. Collier, Elizabeth C. Cooney, Kathryn Coyne, Roberto Docampo, Christopher L. Dupont, Virginia Edgcomb, Elin Einarsson, Pa A. Elustondo, Fernan Federici, Veronica Freire-Beneitez, Nastasia J. Freyria, Kodai Fukuda, Paulo A. Garca, Peter R. Girguis, Fatma Gomaa, Sebastian G. Gornik, Jian Guo, Vladimr Hampl, Yutaka Hanawa, Esteban R. Haro-Contreras, Elisabeth Hehenberger, Andrea Highfield, Yoshihisa Hirakawa, Amanda Hopes, Christopher J. Howe, Ian Hu, Jorge Ibaez, Nicholas A. T. Irwin, Yuu Ishii, Natalia Ewa Janowicz, Adam C. Jones, Ambar Kachale, Konomi Fujimura-Kamada, Binnypreet Kaur, Jonathan Z. Kaye, Eleanna Kazana, Patrick J. Keeling, Nicole King, Lawrence A. Klobutcher, Noelia Lander, Imen Lassadi, Zhuhong Li, Senjie Lin, Jean-Claude Lozano, Fulei Luan, Shinichiro Maruyama, Tamara Matute, Cristina Miceli, Jun Minagawa

Issue&Volume: 2020-04-06

Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

DOI: 10.1038/s41592-020-0796-x

Source: https://www.nature.com/articles/s41592-020-0796-x