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研究发现调控小鼠毛发生长和休息的分子机制
作者:小柯机器人 发布时间:2020/2/28 16:02:33

瑞典卡罗林斯卡学院Maria Kasper团队近日取得一项新成果。他们剖析了小鼠皮肤毛发生长和休息过程的分子机制。相关论文于2020年2月27日在线发表在《细胞-干细胞》杂志上。

研究人员利用单细胞RNA测序和单分子RNA FISH来揭示全厚度皮肤的系统分子图谱、基因表达谱和空间位置,以确定毛发生长和休息期间56种细胞的类型和状态。这些发现揭示了外根鞘(ORS)和内部毛囊层如何协调头发的产生。研究发现ORS由两种混杂但转录上不同的细胞类型组成,这些细胞与基质细胞相互作用的能力不同。内层细胞是在转录上独立于祖细胞的分支,并且每个谱系分化都通过中间态。研究人员还提供了一个在线工具来探索这种全面的皮肤细胞图谱,包括上皮和基质细胞,例如成纤维细胞、血管和免疫细胞,以促进皮肤生物学的进一步发现。

据了解,皮肤动态平衡需要数十种细胞类型精确配合,这些细胞类型共同指导干细胞更新、谱系传承和分化。

附:英文原文

Title: The Molecular Anatomy of Mouse Skin during Hair Growth and Rest

Author: Simon Joost, Karl Annusver, Tina Jacob, Xiaoyan Sun, Tim Dalessandri, Unnikrishnan Sivan, Inês Sequeira, Rickard Sandberg, Maria Kasper

Issue&Volume: 2020-02-27

Abstract: Skin homeostasis is orchestrated by dozens of cell types that together direct stemcell renewal, lineage commitment, and differentiation. Here, we use single-cell RNAsequencing and single-molecule RNA FISH to provide a systematic molecular atlas offull-thickness skin, determining gene expression profiles and spatial locations thatdefine 56 cell types and states during hair growth and rest. These findings revealhow the outer root sheath (ORS) and inner hair follicle layers coordinate hair production.We found that the ORS is composed of two intermingling but transcriptionally distinctcell types with differing capacities for interactions with stromal cell types. Innerlayer cells branch from transcriptionally uncommitted progenitors, and each lineagedifferentiation passes through an intermediate state. We also provide an online toolto explore this comprehensive skin cell atlas, including epithelial and stromal cellssuch as fibroblasts, vascular, and immune cells, to spur further discoveries in skinbiology.

DOI: 10.1016/j.stem.2020.01.012

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30012-6

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx