法国蒙彼利埃大学Jérôme Déjardin、荷兰拉德堡德大学Michiel Vermeulen等研究人员合作总结了对特定染色质位点进行纯化与富集的技术与意义。2020年3月9日,《自然—方法学》在线发表了这一前沿展望论文。
Title: Purification and enrichment of specific chromatin loci
Author: Mathilde Gauchier, Guido van Mierlo, Michiel Vermeulen, Jrme Djardin
Issue&Volume: 2020-03-09
Abstract: Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.
DOI: 10.1038/s41592-020-0765-4
Source: https://www.nature.com/articles/s41592-020-0765-4
Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex