荷兰乌得勒支大学Albert J. R. Heck团队提出基于Orbitrap的单粒子电荷检测质谱法解析异种大分子组装体。2020年3月9日出版的《自然-方法学》杂志发表了这一成果。

他们演示了Orbitrap上的单粒子电荷检测质谱法，用于分析兆尔顿生物分子组装体。他们建立了单个离子的信号幅度随其电荷线性变化的特性，可用于解析混合离子群体，确定电荷状态并从而确定单个离子的质量。这使得能够对多种异种蛋白质组件进行超灵敏分析，包括免疫球蛋白低聚物、核糖体、蛋白质纳米容器和基因组包装的腺相关病毒等。

附：英文原文

Title: Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry

Author: Tobias P. Wrner, Joost Snijder, Antonette Bennett, Mavis Agbandje-McKenna, Alexander A. Makarov, Albert J. R. Heck

Issue&Volume: 2020-03-09

Abstract: We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of individual ions scale linearly with their charge, which can be used to resolve mixed ion populations, determine charge states and thus also determine the masses of individual ions. This enables the ultrasensitive analysis of heterogeneous protein assemblies including immunoglobulin oligomers, ribosomes, proteinaceous nanocontainers and genome-packed adeno-associated viruses.

DOI: 10.1038/s41592-020-0770-7

Source: https://www.nature.com/articles/s41592-020-0770-7