当前位置:科学网首页 > 小柯机器人 >详情
Nov/CCN3能够增强脐带血移植能力
作者:小柯机器人 发布时间:2020/3/21 20:15:35

英国伦敦大学学院Tariq Enver课题组发现,Nov/CCN3通过快速动员人类干细胞活性来增强脐带血(UCB)移植。相关论文于2020年3月19日在线发表在《细胞—干细胞》杂志上。

研究人员发现,用基质细胞调节因子Nov(CCN3)与UCB CD34+细胞进行短暂孵育(8 h),能够将连续移植造血干细胞(HSC)的频率提高6倍。这种快速的反应提示干细胞的动员比扩增重要。
 
因此,在单细胞试验中,Nov可增加表型HSC的克隆形成能力,而不会通过细胞分裂增加其数量。动员与代谢和转录变化都有关,对细胞分裂的追踪表明,克隆形成活动的增加存在于未分裂的细胞中。通过动员的方法来利用干细胞潜能,将有助于理解干细胞的状态转变,并具有向临床转化的意义。
 
据介绍,UCB对幼儿干细胞移植具有很大作用,但其广泛使用受到存量的限制。长期的离体培养提供了一种扩充干细胞和祖细胞来增加移植能力的方法。
 
附:英文原文

Title: Nov/CCN3 Enhances Cord Blood Engraftment by Rapidly Recruiting Latent Human Stem Cell Activity

Author: Rajeev Gupta, Virginia Turati, Duncan Brian, Craig Thrussel, Barry Wilbourn, Gillian May, Tariq Enver

Issue&Volume: 2020-03-19

Abstract: Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and progenitor cells. Here, we show brief incubation (8 h) of UCB CD34+ cells with the matricellular regulator Nov (CCN3) increases the frequency of serially transplantable hematopoietic stem cells (HSCs) 6-fold. This rapid response suggests recruitment rather than expansion of stem cells; accordingly, in single-cell assays, Nov increases the clonogenicity of phenotypic HSCs without increasing their number through cell division. Recruitment is associated with both metabolic and transcriptional changes, and tracing of cell divisions demonstrates that the increased clonogenic activity resides within the undivided fraction of cells. Harnessing latent stem cell potential through recruitment-based approaches will inform understanding of stem cell state transitions with implications for translation to the clinic.

DOI: 10.1016/j.stem.2020.02.012

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30065-5

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx