美国宾夕法尼亚大学Kenneth S. Zaret、Makiko Iwafuchi等研究人员合作揭示，胚胎中的基因网络过渡取决于先驱转录因子和核心组蛋白之间的相互作用。 相关论文于2020年3月16日在线发表在《自然—遗传学》杂志上。
Title: Gene network transitions in embryos depend upon interactions between a pioneer transcription factor and core histones
Author: Makiko Iwafuchi, Isabel Cuesta, Greg Donahue, Naomi Takenaka, Anna B. Osipovich, Mark A. Magnuson, Heinrich Roder, Steven H. Seeholzer, Pilar Santisteban, Kenneth S. Zaret
Abstract: Gene network transitions in embryos and other fate-changing contexts involve combinations of transcription factors. A subset of fate-changing transcription factors act as pioneers; they scan and target nucleosomal DNA and initiate cooperative events that can open the local chromatin. However, a gap has remained in understanding how molecular interactions with the nucleosome contribute to the chromatin-opening phenomenon. Here we identified a short α-helical region, conserved among FOXA pioneer factors, that interacts with core histones and contributes to chromatin opening in vitro. The same domain is involved in chromatin opening in early mouse embryos for normal development. Thus, local opening of chromatin by interactions between pioneer factors and core histones promotes genetic programming.