德国马克斯•普朗克医学研究所Kai Johnsson课题组近日取得一项新成果。他们利用化学遗传学方法改造了纳米抗体。相关论文于2020年2月17日在线发表在《自然—方法学》杂志上。

研究人员介绍了一种工程化的纳米抗体，可以用小分子打开或关闭其对绿色荧光蛋白（GFP）的亲和力。通过控制GFP融合蛋白的细胞定位，研究人员可利用该工程化纳米抗体探索其在基本生物学过程中的作用，这种方法应适用于许多先前描述的GFP融合蛋白。

研究人员还阐明了如何通过小分子控制其他纳米抗体的结合亲和力。

附：英文原文

Title: Chemogenetic Control of Nanobodies

Author: Helen Farrants, Miroslaw Tarnawski, Thorsten G. Mller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Krusslich, Jan Ellenberg, Kai Johnsson

Issue&Volume: 2020-02-17

Abstract: We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

DOI: 10.1038/s41592-020-0746-7

Source: https://www.nature.com/articles/s41592-020-0746-7