当前位置:科学网首页 > 小柯机器人 >详情
大肠杆菌基因组空间结构及相互关系
作者:小柯机器人 发布时间:2020/2/17 10:42:42

美国德克萨斯大学奥斯汀分校Rasika M. Harshey和密歇根大学Peter L. Freddolino合作,通过充分混合的大肠杆菌基因组追踪Mu转座揭示了其广泛联系。该项研究成果在线发表在2020213日的《细胞》上。

染色体的三维结构在细胞调控状态中起着重要作用,被越来越多地认识到。利用噬菌体Mu转座的效率和混杂性来直接测量大肠杆菌中基因组基因座之间的体内相互作用。已经出现了两种总体组织原则:首先,染色体是充分混合且没有隔室的,在所有测定的基因座之间自由发生转座。第二,几个基因家族/区域表现出聚集:无论线性基因组距离如何,都会出现强三维共定位。SMC /凝缩蛋白MukB和核凝缩蛋白亚基HU-α的活性对于充分混合状态是必不可少的。HU-α对于6/7核糖体RNA编码基因座的聚集也是必需的。

模型可以解释数据,该模型中的染色体结构由DNA复制和染色体松弛之间的动态竞争驱动,为确定区域特异性如何对染色体结构和基因调控做出贡献提供了基础。

附:英文原文

Title: A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition

Author: David M. Walker, Peter L. Freddolino, Rasika M. Harshey

Issue&Volume: February 13, 2020

Abstract: The three-dimensional structures of chromosomes are increasingly being recognizedas playing a major role in cellular regulatory states. The efficiency and promiscuityof phage Mu transposition was exploited to directly measure in vivo interactions between genomic loci in E. coli. Two global organizing principles have emerged: first, the chromosome is well-mixedand uncompartmentalized, with transpositions occurring freely between all measuredloci; second, several gene families/regions show “clustering”: strong three-dimensionalco-localization regardless of linear genomic distance. The activities of the SMC/condensinprotein MukB and nucleoid-compacting protein subunit HU-α are essential for the well-mixedstate; HU-α is also needed for clustering of 6/7 ribosomal RNA-encoding loci. Thedata are explained by a model in which the chromosomal structure is driven by dynamiccompetition between DNA replication and chromosomal relaxation, providing a foundationfor determining how region-specific properties contribute to both chromosomal structureand gene regulation.

DOI: 10.1016/j.cell.2020.01.031

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30112-4

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/