美国波士顿大学Devin R. Beaulieu研究组研究提出反射双光子显微镜可同时进行多平面成像。该项研究成果2020年2月10日在线发表在《自然—方法学》上。

他们通过执行近乎瞬时的轴向扫描，同时保持三维微米水平的分辨率，展示了双光子显微镜的视频速率多平面成像。他们的技术被称为发射显微镜，可以在较大深度范围内监视神经元种群，并且可以作为传统设计的简单附件来实现。

据悉，多光子显微镜因其具有能够提供散射组织深处的高分辨率图像的独特能力而获得了极大的普及。

附：英文原文

Title: Simultaneous multiplane imaging with reverberation two-photon microscopy

Author: Devin R. Beaulieu, Ian G. Davison, Kvlcm Kl, Thomas G. Bifano, Jerome Mertz

Issue&Volume: 2020-02-10

Abstract: Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.

DOI: 10.1038/s41592-019-0728-9

Source: https://www.nature.com/articles/s41592-019-0728-9