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肥大细胞蛋白质组分析显示其与过敏相关组成
作者:小柯机器人 发布时间:2020/2/13 16:15:29

德国癌症研究中心Hans-Reimer Rodewald研究组发现人类肥大细胞蛋白质组显示出细胞消融的独特谱系、假定功能和结构基础。这一研究成果在线发表在2020年2月11日的国际学术期刊《免疫》上。

为了解主要肥大细胞功能的结构基础,他们通过定量质谱分析了原代人和小鼠肥大细胞的蛋白质组他们鉴定了肥大细胞特有的蛋白质组特征,其具独特的谱系,仅与其他免疫细胞类型(包括先天免疫细胞)远缘相关。人与小鼠之间的蛋白质组比较表明核心肥大细胞功能的进化保守性。除了与脱粒和蛋白聚糖生物合成相关的特定蛋白酶和蛋白质外,肥大细胞还表达可能与神经元和神经递质代谢相互作用相关的蛋白质,包括细胞粘附分子、离子通道和G蛋白偶联受体。为了研究严重过敏性疾病中的靶向细胞消融,他们在人类皮肤活检中使用MRGPRX2消除肥大细胞。这些蛋白质组学分析表明,肥大细胞在免疫系统中可能具有独特的作用,可能与神经系统交织在一起。

研究人员表示,肥大细胞是罕见的与人过敏有关的组织驻留细胞。

附:英文原文

Title: Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation

Author: Thomas Plum, Xi Wang, Mandy Rettel, Jeroen Krijgsveld, Thorsten B. Feyerabend, Hans-Reimer Rodewald

Issue&Volume: February 11, 2020

Abstract: Mast cells are rare tissue-resident cells of importance to human allergies. To understandthe structural basis of principle mast cell functions, we analyzed the proteome ofprimary human and mouse mast cells by quantitative mass spectrometry. We identifieda mast-cell-specific proteome signature, indicative of a unique lineage, only distantlyrelated to other immune cell types, including innate immune cells. Proteome comparisonbetween human and mouse suggested evolutionary conservation of core mast cell functions.In addition to specific proteases and proteins associated with degranulation and proteoglycanbiosynthesis, mast cells expressed proteins potentially involved in interactions withneurons and neurotransmitter metabolism, including cell adhesion molecules, ion channels,and G protein coupled receptors. Toward targeted cell ablation in severe allergicdiseases, we used MRGPRX2 for mast cell depletion in human skin biopsies. These proteomeanalyses suggest a unique role of mast cells in the immune system, probably intertwinedwith the nervous system.

DOI: 10.1016/j.immuni.2020.01.012

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30039-X

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新if:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx