美国匹兹堡大学Yi Shi等研究人员合作开发出新型高效纳米抗体用于中和SARS-CoV-2。2020年11月5日，《科学》杂志在线发表了这项成果。

Title: Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2

Author: Yufei Xiang, Sham Nambulli, Zhengyun Xiao, Heng Liu, Zhe Sang, W. Paul Duprex, Dina Schneidman-Duhovny, Cheng Zhang, Yi Shi

Issue&Volume: 2020/11/05

Abstract: AbstractCost-effective, efficacious therapeutics are urgently needed against the COVID-19 pandemic. Here, we used camelid immunization and proteomics to identify a large repertoire of highly potent neutralizing nanobodies (Nbs) to the SARS-CoV-2 spike (S) protein receptor-binding domain (RBD). We discovered Nbs with picomolar to femtomolar affinities that inhibit viral infection at sub-ng/ml concentration and determined a structure of one of the most potent in complex with RBD. Structural proteomics and integrative modeling revealed multiple distinct and non-overlapping epitopes and indicated an array of potential neutralization mechanisms. We constructed multivalent Nb constructs that achieved ultrahigh neutralization potency (IC50s as low as 0.058 ng/ml) and may prevent mutational escape. These thermostable Nbs can be rapidly produced in bulk from microbes and resist lyophilization, and aerosolization.

DOI: 10.1126/science.abe4747

Source: https://science.sciencemag.org/content/early/2020/11/04/science.abe4747