加拿大大学健康网络Gordon M. Keller、Alexander A. Mikryukov课题组发现，BMP10信号促进人多能干细胞（hPSC）衍生心血管祖细胞发育为心内膜细胞。相关论文在线发表在2020年11月2号出版的《细胞-干细胞》杂志上。

为了获取人心脏内膜细胞，研究人员利用了hPSC衍生的内皮细胞，该细胞显示出许多心内膜特征，包括表达体内该谱系细胞的基因组、在体外诱导未成熟心肌细胞分化为小梁心肌、以及具有内皮向间充质细胞转化的能力。对hPSC衍生心内膜细胞发育所需信号通路的分析确定了BMP10在该过程中的新功能。

据了解，胚胎内膜对于早期心脏的发育至关重要，因为它诱导小梁心肌（形成的第一个心脏组织）的生成，并且是构成瓣膜和部分冠状脉管系统细胞的来源。凭借这种分化潜能，人心脏内膜细胞可以提供独特的治疗潜能，包括工程化生物瓣膜和基于细胞的治疗策略，以取代受损心脏中的冠脉血管。

附：英文原文

Title: BMP10 Signaling Promotes the Development of Endocardial Cells from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitors

Author: Alexander A. Mikryukov, Amine Mazine, Bei Wei, Donghe Yang, Yifei Miao, Mingxia Gu, Gordon M. Keller

Issue&Volume: 2020-11-02

Abstract: The embryonic endocardium is essential for early heart development as it functionsto induce trabecular myocardium, the first heart tissue to form, and is the sourceof the cells that make up the valves and a portion of the coronary vasculature. Withthis potential, human endocardial cells could provide unique therapeutic opportunitiesthat include engineering biological valves and cell-based therapy strategies to replacecoronary vasculature in damaged hearts. To access human endocardial cells, we generateda human pluripotent stem cell (hPSC)-derived endothelial population that displaysmany characteristics of endocardium, including expression of the cohort of genes thatidentifies this lineage in vivo, the capacity to induce a trabecular fate in immature cardiomyocytes in vitro, and the ability to undergo an endothelial-to-mesenchymal transition. Analyses ofthe signaling pathways required for development of the hPSC-derived endocardial cellsidentified a novel role for BMP10 in the specification of this lineage from cardiovascular mesoderm.

DOI: 10.1016/j.stem.2020.10.003

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30497-5