近日，美国国立卫生研究院Rémy Bosselut及其小组绘制出小鼠和人类αβT细胞发育的表观基因组和转录组整合图谱。该研究于2020年11月25日在线发表于国际一流学术期刊《免疫》。

Title: An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development

Author: Laura B. Chopp, Vishaka Gopalan, Thomas Ciucci, Allison Ruchinskas, Zachary Rae, Manon Lagarde, Yayi Gao, Caiyi Li, Marita Bosticardo, Francesca Pala, Ferenc Livak, Michael C. Kelly, Sridhar Hannenhalli, Rémy Bosselut

Issue&Volume: 2020-11-25

Abstract: αβ lineage T cells, most of which are CD4+ or CD8+ and recognize MHC I- or MHC II-presented antigens, are essential for immune responsesand develop from CD4+CD8+ thymocytes. The absence of in vitro models and the heterogeneity of αβ thymocytes have hampered analyses of their intrathymicdifferentiation. Here, combining single-cell RNA and ATAC (chromatin accessibility)sequencing, we identified mouse and human αβ thymocyte developmental trajectories.We demonstrated asymmetric emergence of CD4+ and CD8+ lineages, matched differentiation programs of agonist-signaled cells to their MHCspecificity, and identified correspondences between mouse and human transcriptomicand epigenomic patterns. Through computational analysis of single-cell data and bindingsites for the CD4+-lineage transcription factor Thpok, we inferred transcriptional networks associatedwith CD4+- or CD8+-lineage differentiation, and with expression of Thpok or of the CD8+-lineage factor Runx3. Our findings provide insight into the mechanisms of CD4+ and CD8+ T cell differentiation and a foundation for mechanistic investigations of αβ T celldevelopment.

DOI: 10.1016/j.immuni.2020.10.024

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30465-9