美国华盛顿州立大学Yuehe Lin课题组的一项最新研究开发出生物共反应物增强的电化学发光显微镜观察细胞内结构和转运。 这一研究成果发表在2020年11月14日出版的国际学术期刊《德国应用化学》上。

一种生物共反应物增强的电化学发光(ECL)显微镜揭示了细胞内结构和动态转运。该显微镜技术使用Ru(bpy)₃²⁺作为连接细胞外和细胞内环境的电化学分子天线，并通过“催化途径”使用细胞内生物分子作为ECL反应的共反应物。因此，细胞内结构的识别无需使用多种标签，并且涉及DNA氧化损伤的自噬是由核ECL信号确定的。

时间分辨图像序列揭示了由于细胞膜几何性质对诱导跨膜电压的影响，细胞电穿孔的普遍边缘效应。Ru(bpy)₃³⁺在不同细胞间室的动态转运显示了与肌动蛋白骨架相关的细胞内扩散率的不均一性。除了单细胞研究，生物共反应物增强ECL显微镜还扩展到对其他生物的成像。

附：英文原文

Title: Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport

Author: Cheng Ma, Shaojun Wu, Yang Zhou, Hui-Fang Wei, Jianrong Zhang, Zixuan Chen, Jun-Jie Zhu, Yuehe Lin, Wenlei Zhu

Issue&Volume: 14 November 2020

Abstract: A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and the autophagy involving DNA oxidative damage is determined by nuclear ECL signals. Time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is expanded to image other organisms.

DOI: 10.1002/anie.202012171

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202012171