美国耶鲁大学樊荣研究团队开发出新型高分辨空间组学技术。这一研究成果于2020年11月13日在线发表在国际学术期刊《细胞》上。
Title: High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue
Author: Yang Liu, Mingyu Yang, Yanxiang Deng, Graham Su, Archibald Enninful, Cindy C. Guo, Toma Tebaldi, Di Zhang, Dongjoo Kim, Zhiliang Bai, Eileen Norris, Alisia Pan, Jiatong Li, Yang Xiao, Stephanie Halene, Rong Fan
Issue&Volume: 2020-11-13
Abstract: We present deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq)for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via next-generationsequencing (NGS). Parallel microfluidic channels were used to deliver DNA barcodesto the surface of a tissue slide, and crossflow of two sets of barcodes, A1-50 andB1-50, followed by ligation in situ, yielded a 2D mosaic of tissue pixels, each containing a unique full barcode AB.Application to mouse embryos revealed major tissue types in early organogenesis aswell as fine features like microvasculature in a brain and pigmented epithelium inan eye field. Gene expression profiles in 10-μm pixels conformed into the clustersof single-cell transcriptomes, allowing for rapid identification of cell types andspatial distributions. DBiT-seq can be adopted by researchers with no experience inmicrofluidics and may find applications in a range of fields including developmentalbiology, cancer biology, neuroscience, and clinical pathology.
DOI: 10.1016/j.cell.2020.10.026
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31390-8