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研究揭示角质形成细胞对鼓膜维持的贡献
作者:小柯机器人 发布时间:2020/11/15 0:24:07

美国加州大学旧金山分校Aaron D. Tward团队揭示角质形成细胞对鼓膜维持的贡献。这一研究成果于2020年11月11日在线发表在国际学术期刊《细胞—干细胞》上。

使用单细胞RNA测序、谱系追踪、全器官外植体和活细胞成像,研究人员发现稳态的鼓膜(TM)表皮不同于其他表皮部位,并且具有离散的增生区以及多个角质形成细胞群的三维层次结构。TM干细胞位于上级TM的离散位置,并产生长寿命的克隆和定型祖细胞(CP)。
 
CP克隆表现出侧向迁移,其增殖能力得到Pdgfra+成纤维细胞的支持,从而产生迁移但非增殖的后代。人类TM的单细胞测序显示出相似的细胞类型和转录程序。因此,在稳态过程中,TM角质形成细胞通过增生的CP状态转运并表现出定向的横向迁移。这项工作为理解TM疾病和角质形成细胞建模奠定了基础。
 
据介绍,TM对于听力至关重要,并需要对细胞碎片的连续清除,但对TM表皮中的稳态机制知之甚少。
 
附:英文原文

Title: A Hierarchy of Proliferative and Migratory Keratinocytes Maintains the Tympanic Membrane

Author: Stacey M. Frumm, Shengyang Kevin Yu, Joseph Chang, Jordan A. Artichoker, Sonia M. Scaria, Katharine P. Lee, Lauren E. Byrnes, Julie B. Sneddon, Aaron D. Tward

Issue&Volume: 2020-11-11

Abstract: The tympanic membrane (TM) is critical for hearing and requires continuous clearingof cellular debris, but little is known about homeostatic mechanisms in the TM epidermis.Using single-cell RNA sequencing, lineage tracing, whole-organ explant, and live-cellimaging, we show that homeostatic TM epidermis is distinct from other epidermal sitesand has discrete proliferative zones with a three-dimensional hierarchy of multiplekeratinocyte populations. TM stem cells reside in a discrete location of the superiorTM and generate long-lived clones and committed progenitors (CPs). CP clones exhibitlateral migration, and their proliferative capacity is supported by Pdgfra+ fibroblasts,generating migratory but non-proliferative progeny. Single-cell sequencing of thehuman TM revealed similar cell types and transcriptional programming. Thus, duringhomeostasis, TM keratinocytes transit through a proliferative CP state and exhibitdirectional lateral migration. This work forms a foundation for understanding TM disordersand modeling keratinocyte biology.

DOI: 10.1016/j.stem.2020.10.006

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30500-2

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx