德国波恩大学的Jeroen Sidney Dickschat研究团队经过不懈努力阐明了一种嗜酸细小链胞菌中的二萜生物合成酶（Catenul‐14‐en‐6‐ol Synthase）的反应机理。2020年11月10日出版的《德国应用化学》杂志发表了这一最新研究成果。

在该研究中，团队发现了一种新的来自放线菌目的嗜酸细小链孢菌的二萜合成酶，并揭示了其产物的结构，包括通过手性选择性氘代法确定了产物的绝对构象。为了进一步研究这个阳离子萜烯环化串联反应的机理，研究人员通过引入同位素标记的底物和部分影响活性的底物类似物，使得该酶合成了各种偏离反应路线的产物，这些产物为了解串联反应中的中间体提供了更多视角。

在了解了该反应机理之后，研究人员利用该酶仿生合成了一种来自红色海藻Laurencia microcladia中已知的溴代倍半萜的二萜类似物。

附：英文原文

Title: Diterpene Biosynthesis in Catenulispora acidiphila: On the Mechanism of Catenul‐14‐en‐6‐ol Synthase

Author: Geng Li, Yue-Wei Guo, Jeroen Sidney Dickschat

Issue&Volume: 10 November 2020

Abstract: A new diterpene synthase from the actinomycete Catenulispora acidiphila was identified and the structures of its products were elucidated, including the absolute configurations by an enantioselective deuteration approach. The mechanism of the cationic terpene cyclisation cascade was deeply studied through the use of isotopically labelled substrates and of substrate analogs with partially blocked reactivity, resulting in derailment products that gave further insights into the intermediates along the cascade. Their chemistry was studied, leading to the biomimetic synthesis of a diterpenoid analog of a brominated sesquiterpene known from the red seaweed Laurencia microcladia.

DOI: 10.1002/anie.202014180

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202014180