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科学家在类原肠胚中实现心脏的发生
作者:小柯机器人 发布时间:2020/11/11 22:52:57

近日,瑞士洛桑联邦理工学院Matthias P. Lutolf及其研究组在类原肠胚中实现心脏的发生。2020年11月10日,国际知名学术期刊《细胞—干细胞》在线发表了这一成果。

研究人员发现,小鼠胚胎干细胞(mESC)可以被诱导,进而以体内类似的时空保真度稳健地经历早期心脏器官发生的基本步骤。这些轴向分化的胚胎类器官(类原肠胚)模拟了胚胎发育并支持心血管祖细胞的生成,包括第一和第二心脏区域。在原肠样管附近形成跳动的心脏组织之前,心脏祖细胞自组织成一个新月形的前部区域,从而心脏组织被心内膜样层隔开。

这些发现揭示了mESC通过多种组织的协调发育来执行器官发生的惊人潜力。这个平台可能是研究心脏发育的绝佳工具,它具有前所未有的细节和通量。

据介绍,类器官是研究组织发育、生理和疾病的强大模型。然而,当前的培养系统破坏了天然器官发生的复杂形态发生过程所需的诱导性组织-组织相互作用。

附:英文原文

Title: Capturing Cardiogenesis in Gastruloids

Author: Giuliana Rossi, Nicolas Broguiere, Matthew Miyamoto, Andrea Boni, Romain Guiet, Mehmet Girgin, Robert G. Kelly, Chulan Kwon, Matthias P. Lutolf

Issue&Volume: 2020-11-10

Abstract: Organoids are powerful models for studying tissue development, physiology, and disease.However, current culture systems disrupt the inductive tissue-tissue interactionsneeded for the complex morphogenetic processes of native organogenesis. Here, we showthat mouse embryonic stem cells (mESCs) can be coaxed to robustly undergo fundamentalsteps of early heart organogenesis with an in-vivo-like spatiotemporal fidelity. These axially patterned embryonic organoids (gastruloids)mimic embryonic development and support the generation of cardiovascular progenitors,including first and second heart fields. The cardiac progenitors self-organize intoan anterior domain reminiscent of a cardiac crescent before forming a beating cardiactissue near a putative primitive gut-like tube, from which it is separated by an endocardial-likelayer. These findings unveil the surprising morphogenetic potential of mESCs to executekey aspects of organogenesis through the coordinated development of multiple tissues.This platform could be an excellent tool for studying heart development in unprecedented detail and throughput.

DOI: 10.1016/j.stem.2020.10.013

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30507-5

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx