近日，英国阿伯丁大学Hai Deng及其研究团队发现并生物合成了一种新型抗菌脱水非核糖体三肽。2020年10月27日，国际知名学术期刊《德国应用化学》在线发表了这一成果。

据研究人员介绍，脱氢丙氨酸（Dha）和脱氢丁胺（Dhb）在各种化学和生物反应中显示出相当大的灵活性。含有Dha和/或Dhb残留物的天然产物经常被发现具有多种生物活性。虽然(Z)-构象在自然界中占主导地位，但到目前为止，仅发现了少数包含(E)-Dhb的代谢产物。

Title: Discovery and biosynthetic investigation of a new antibacterial dehydrated non‐ribosomal tripeptide

Author: Shan Wang, Qing Fang, ZHOU LU, Yingli GAO, Laurent Trembleau, Rainer Ebel, Jeanette H Andersen, Carol Philips, Samantha Law, Hai Deng

Issue&Volume:

Abstract: Dehydroalanine (Dha) and dehydrobutyrine (Dhb) display considerable flexibility in a variety of chemical and biological reactions. Natural products containing Dha and/or Dhb residues are often found to possess diverse biological activities. While the (  Z  )‐geometry is predominant in nature, only a handful of metabolites containing (  E  )‐Dhb have been found thus far. Here we report discovery of a new antimicrobial peptide, albopeptide, through NMR analysis and chemical synthesis, which contains two contiguous unsaturated residues, Dha‐(  E  )‐Dhb. It displays narrow‐spectrum activity against vancomycin‐resistant  Enterococcus faecium.  In vitro  biochemical assays show that albopeptide originates from a noncanonical NRPS pathway, featured with dehydration processes, catalysed by unusual condensation domains. Finally, we provide evidence of the occurrence of a previously untapped group of short unsaturated peptides in the bacterial kingdom, suggesting an important biological function in bacteria.

DOI: 10.1002/anie.202012902

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202012902