Title: Engineering Fe–N Doped Graphene to Mimic Biological Functions of NADPH Oxidase in Cells
Author: Di Wu, Jingkun Li, Shujuan Xu, Qianqian Xie, Yanxia Pan, Xi Liu, Ronglin Ma, Huizhen Zheng, Meng Gao, Weili Wang, Jia Li, Xiaoming Cai, Frédéric Jaouen, Ruibin Li
Issue&Volume: October 27, 2020
Abstract: NADPH oxidase (NOX) as a transmembrane enzyme complex controls the generation of superoxide that plays important roles in immune signaling pathway. NOX inactivation may elicit immunodeficiency and cause chronic granulomatous disease (CGD). Biocompatible synthetic materials with NOX-like activities would therefore be interesting as curative and/or preventive approaches in case of NOX deficiency. Herein, we synthesized a Fe–N doped graphene (FeNGR) nanomaterial that could mimic the activity of NOX by efficiently catalyzing the conversion of NADPH into NADP+ and triggering the generation of oxygen radicals. The resulting FeNGR nanozyme had similar cellular distribution to NOX and is able to mimic the enzyme function in NOX-deficient cells by catalyzing the generation of superoxide and retrieving the immune activity, evidenced by TNF-α, IL-1β, and IL-6 production in response to Alum exposure. Overall, our study discovered a synthetic material (FeNGR) to mimic NOX and demonstrated its biological function in immune activation of NOX-deficient cells.
DOI: 10.1021/jacs.0c08360
Source: https://pubs.acs.org/doi/10.1021/jacs.0c08360
JACS:《美国化学会志》,创刊于1879年。隶属于美国化学会,最新IF:14.612
官方网址:https://pubs.acs.org/journal/jacsat
投稿链接:https://acsparagonplus.acs.org/psweb/loginForm?code=1000