美国加州大学洛杉矶分校Ellen M. Sletten、德国亥姆霍兹联合会Oliver T. Bruns等研究人员合作开发出与激发激光匹配的短波红外聚次甲基荧光团，从而实现无创、多色体内实时成像。相关论文于2020年10月19日在线发表于国际学术期刊《自然—化学》。
Title: Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time
Author: Emily D. Cosco, Anthony L. Spearman, Shyam Ramakrishnan, Jakob G. P. Lingg, Mara Saccomano, Monica Pengshung, Bernardo A. Ars, Kelly C. Y. Wong, Sarah Glasl, Vasilis Ntziachristos, Martin Warmer, Ryan R. McLaughlin, Oliver T. Bruns, Ellen M. Sletten
Abstract: High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial scattering and autofluorescence in tissue at visible (350–700 nm) and near-infrared (700–1,000 nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000–2,000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multicolour SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1,064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-colour imaging with high temporal and spatial resolutions.