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新型短波红外荧光团实现无创多色的体内实时成像
作者:小柯机器人 发布时间:2020/10/28 12:46:41

美国加州大学洛杉矶分校Ellen M. Sletten、德国亥姆霍兹联合会Oliver T. Bruns等研究人员合作开发出与激发激光匹配的短波红外聚次甲基荧光团,从而实现无创、多色体内实时成像。相关论文于2020年10月19日在线发表于国际学术期刊《自然—化学》。

通过针对短波红外(SWIR,1,000–2,000 nm)区域光学对比剂的设计以及成像技术的补充发展,研究人员实现了对动物的实时无创多色成像实验。研究人员开发了可调节的SWIR发射型flavylium polymethine 染料,并为此类明亮的SWIR对比剂建立了结构与光物理性质之间的关系。同时,研究人员设计了具有可变近红外/ SWIR激发和单通道检测功能的成像系统,从而可促进光学引导手术的多色SWIR成像以及具有多重检测功能的活动小鼠成像。经过优化的染料与980 nm和1,064 nm激光相匹配,并结合了临床认可的吲哚菁绿,进而实现了具有高时间和空间分辨率的实时三色成像。
 
据介绍,高分辨率、多路复用实验是细胞成像的主要手段。然而,由于在可见光(350–700 nm)和近红外(700–1,000 nm)波长下组织中的大量散射和自发荧光,在动物中进行类似的实验具有挑战性。
 
附:英文原文

Title: Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time

Author: Emily D. Cosco, Anthony L. Spearman, Shyam Ramakrishnan, Jakob G. P. Lingg, Mara Saccomano, Monica Pengshung, Bernardo A. Ars, Kelly C. Y. Wong, Sarah Glasl, Vasilis Ntziachristos, Martin Warmer, Ryan R. McLaughlin, Oliver T. Bruns, Ellen M. Sletten

Issue&Volume: 2020-10-19

Abstract: High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial scattering and autofluorescence in tissue at visible (350–700 nm) and near-infrared (700–1,000 nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000–2,000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multicolour SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1,064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-colour imaging with high temporal and spatial resolutions.

DOI: 10.1038/s41557-020-00554-5

Source: https://www.nature.com/articles/s41557-020-00554-5

期刊信息

Nature Chemistry:《自然—化学》,创刊于2009年。隶属于施普林格·自然出版集团,最新IF:21.687
官方网址:https://www.nature.com/nchem/
投稿链接:https://mts-nchem.nature.com/cgi-bin/main.plex