新加坡南洋理工大学Yanli Zhao等研究人员开发出自组装单位点纳米酶，可用于肿瘤特异性扩增级联酶促疗法。这一研究成果于2020年10月22日在线发表于国际学术期刊《德国应用化学》。

Title: Self‐Assembled Single‐Site Nanozyme for Tumor‐Specific Amplified Cascade Enzymatic Therapy

Author: Yanli Zhao, Dongdong Wang, Huihui Wu, Changlai Wang, Long Gu, Hongzhong Chen, Deblin Jana, Xueying Wang, Pengping Xu, Zhen Guo, Qianwang Chen, Lili Feng, Jiawei Liu

Issue&Volume:

Abstract: Nanomaterials with enzyme‐mimicking activity (nanozyme) are at the targeted frontier for therapeutic interventions. However, it still remains a formidable challenge to selectively kill tumor cells through enzymatic reactions, while leaving normal cells unharmed. Herein, a new strategy is presented by developing single‐site cascade enzymatic reaction for tumor‐specific therapy while avoiding off‐target toxicity to normal tissues. Copper hexacyanoferrate (Cu‐HCF) nanozyme with active single‐site copper exhibits cascade enzymatic activity (i.e., glutathione oxidase and peroxidase) within tumor microenvironment. Results show that Cu‐HCF single‐site nanozymes (SSNEs) first exert tumor‐specific glutathione oxidase ability through depleting intracellular glutathione and converting single‐site CuII species to CuI for subsequent amplified peroxidase activity via Fenton‐type Harber‐Weiss reaction, and thus substantial highly toxic hydroxyl radicals are generated for tumor cell apoptosis. Our work demonstrates that SSNEs could amplify the killing efficacy of singlet oxygen species and suppress the tumor growth in vivo, providing a promising self‐assembled single‐site nanozyme for amplified cascade enzymatic anticancer application.

DOI: 10.1002/anie.202008868

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202008868