北京大学李毓龙等研究人员合作开发出可用于监测体内多巴胺能活性的下一代GRAB传感器。该研究于2020年10月21日在线发表于国际一流学术期刊《自然—方法学》。

Title: Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

Author: Fangmiao Sun, Jingheng Zhou, Bing Dai, Tongrui Qian, Jianzhi Zeng, Xuelin Li, Yizhou Zhuo, Yajun Zhang, Yipan Wang, Cheng Qian, Ke Tan, Jiesi Feng, Hui Dong, Dayu Lin, Guohong Cui, Yulong Li

Issue&Volume: 2020-10-21

Abstract: Dopamine (DA) plays a critical role in the brain, and the ability to directly measure dopaminergic activity is essential for understanding its physiological functions. We therefore developed red fluorescent G-protein-coupled receptor-activation-based DA (GRABDA) sensors and optimized versions of green fluorescent GRABDA sensors. In response to extracellular DA, both the red and green GRABDA sensors exhibit a large increase in fluorescence, with subcellular resolution, subsecond kinetics and nanomolar-to-submicromolar affinity. Moreover, the GRABDA sensors resolve evoked DA release in mouse brain slices, detect evoked compartmental DA release from a single neuron in live flies and report optogenetically elicited nigrostriatal DA release as well as mesoaccumbens dopaminergic activity during sexual behavior in freely behaving mice. Coexpressing red GRABDA with either green GRABDA or the calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity in distinct circuits in vivo. Red and improved green versions of the genetically encoded dopamine sensor GRABDA have been developed. These neurotransmitter sensors are used alone or in combination with, for example, calcium sensors in behaving fruit flies and rodents.

DOI: 10.1038/s41592-020-00981-9

Source: https://www.nature.com/articles/s41592-020-00981-9