北京大学汤富酬、付卫等研究人员合作利用单细胞多组学测序，揭示出人类结直肠癌肿瘤基质细胞中普遍的基因组改变。这一研究成果于2020年10月22日在线发表在国际学术期刊《癌细胞》上。

Title: Single-Cell Multiomics Sequencing Reveals Prevalent Genomic Alterations in Tumor Stromal Cells of Human Colorectal Cancer

Author: Yuan Zhou, Shuhui Bian, Xin Zhou, Yueli Cui, Wendong Wang, Lu Wen, Limei Guo, Wei Fu, Fuchou Tang

Issue&Volume: 2020-10-22

Abstract: To what extent stromal cells in the tumor microenvironment (TME) are transformed bycolorectal cancer (CRC) cells is unexplored. To dissect alterations in these non-malignantcells, we performed single-cell multiomics sequencing of 21 patients with microsatellite-stableCRCs and 6 cancer-free, elderly individuals. Surprisingly, somatic copy number alterations(SCNAs) are prevalent in immune cells, fibroblasts, and endothelial cells in boththe TME and the normal tissues of each individual. Moreover, the proportions of fibroblastswith SCNAs in tumors (11.1%–47.7%) are much higher than those in adjacent normal tissues(1.1%–10.6%), with gain of chromosome 7 strongly enriched in the TME, clearly indicatingclonal expansion. Furthermore, five genes (BGN, RCN3, TAGLN, MYL9, and TPM2) are identified as fibroblast-specific biomarkers of poorer prognosis of CRC. Ourstudy provides evidence and functional relevance of pervasive genomic alterationsin the stromal cells of TME in CRC.

DOI: 10.1016/j.ccell.2020.09.015

Source: https://www.cell.com/cancer-cell/fulltext/S1535-6108(20)30489-X