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一体式灌注机能够将损伤的肝脏保存1周
作者:小柯机器人 发布时间:2020/1/16 10:36:24

一体式灌注机能够将损伤的肝脏保存1周,这一成果由瑞士苏黎世联邦理工学院Pierre-Alain Clavien团队近期取得。相关论文2020年1月13日在线发表于国际学术期刊《自然—生物技术》。

研究人员报告了一种肝脏灌注机,该机器集成了多种核心生理功能,包括葡萄糖水平和加氧的自动管理、废物清除和血细胞比容控制。研究人员使用猪肝逐步开发了该机器。对多种离体参数和体内早期再灌注的研究证明了灌注1周的猪肝活力,且无需额外的血液制品或灌注液交换。研究人员对所有欧洲中心拒绝移植的十个受伤的人类肝脏进行了测试。灌注7天后,六个人的肝脏显示出胆汁生成、凝血因子合成、维持的细胞能量(ATP)和完整的肝结构,从而表明肝功能得以保留。

据介绍,能够将代谢活跃的肝脏离体保存1周或更长时间的能力可以修复损伤的肝脏,否则这些肝脏将无法移植。当前的常温灌注方法只能将人类肝脏保存24小时。

附:英文原文

Title: An integrated perfusion machine preserves injured human livers for 1 week

Author: Dilmurodjon Eshmuminov, Dustin Becker, Lucia Bautista Borrego, Max Hefti, Martin J. Schuler, Catherine Hagedorn, Xavier Muller, Matteo Mueller, Christopher Onder, Rolf Graf, Achim Weber, Philipp Dutkowski, Philipp Rudolf von Rohr, Pierre-Alain Clavien

Issue&Volume: 2020-01-13

Abstract: The ability to preserve metabolically active livers ex vivo for 1 week or more could allow repair of poor-quality livers that would otherwise be declined for transplantation. Current approaches for normothermic perfusion can preserve human livers for only 24h. Here we report a liver perfusion machine that integrates multiple core physiological functions, including automated management of glucose levels and oxygenation, waste-product removal and hematocrit control. We developed the machine in a stepwise fashion using pig livers. Study of multiple ex vivo parameters and early phase reperfusion in vivo demonstrated the viability of pig livers perfused for 1 week without the need for additional blood products or perfusate exchange. We tested the approach on ten injured human livers that had been declined for transplantation by all European centers. After a 7-d perfusion, six of the human livers showed preserved function as indicated by bile production, synthesis of coagulation factors, maintained cellular energy (ATP) and intact liver structure.

DOI: 10.1038/s41587-019-0374-x

Source: https://www.nature.com/articles/s41587-019-0374-x

期刊信息

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:31.864
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex