德国欧洲分子生物学实验室（EMBL）的Robert Prevedel和Alba Diz-Muñoz等研究人员合作综述了生物力学研究中一个新兴的工具——布里渊显微镜。该文章2019年9月23日在线发表于《自然—方法学》。

研究人员通过描述基本的生物物理原理并讨论由异质生物物质引起的布里渊光谱的解释来回顾这项新兴技术。研究人员将进一步阐述该技术的局限性及其在生物学领域获得见识的潜力，以指导来自各个领域对此感兴趣的研究人员。

据介绍，细胞和组织的机械特性在细胞功能、发育和疾病中的作用和重要性已得到广泛认可，但是目前用于评估它们的标准技术表现出内在的局限性。最近，布里渊显微术是一种光学弹性成像技术，它已发展成为一种无损、无标记和无接触的方法，可以以3D衍射极限分辨率探测生物样品的粘弹性。这引起了生物学和医学研究界的更多关注，但同时也引发了有关所测物理量的解释和相关性的辩论。

附：英文原文

Title: Brillouin microscopy: an emerging tool for mechanobiology

Author: Robert Prevedel, Alba Diz-Muoz, Giancarlo Ruocco, Giuseppe Antonacci

Issue&Volume: 2019-09-23

Abstract: 

The role and importance of mechanical properties of cells and tissues in cellular function, development and disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations. Recently, Brillouin microscopy, a type of optical elastography, has emerged as a non-destructive, label- and contact-free method that can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This led to increased attention amongst the biological and medical research communities, but it also sparked debates about the interpretation and relevance of the measured physical quantities. Here, we review this emerging technology by describing the underlying biophysical principles and discussing the interpretation of Brillouin spectra arising from heterogeneous biological matter. We further elaborate on the technique’s limitations, as well as its potential for gaining insights in biology, in order to guide interested researchers from various fields.

DOI: 10.1038/s41592-019-0543-3

Source:https://www.nature.com/articles/s41592-019-0543-3