韩国科学技术学院Won Do Heo和Byung Ouk Park等研究人员合作研发了光遗传激活的细胞内抗体，从而用于直接调节内源性蛋白。相关论文2019年10月14日在线发表在《自然—方法学》上。

研究人员报道了由分开的抗体片段和蓝光诱导性异源二聚化结构域组成的光遗传学激活的细胞内抗体（光抗体）。研究人员通过从先前开发的细胞内抗体中产生若干种抗体来扩展了这种光抗体平台，并证明了凝溶胶蛋白和β2-肾上腺素能受体（β2AR）光抗体的光激活分别抑制了内源性凝溶胶蛋白活性和β2AR信号传导。

研究人员表示，细胞内抗体已成为用于成像、调节和中和内源性靶蛋白的强大工具。

附：英文原文

Title: Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins

Author: Daseuli Yu, Hansol Lee, Jongryul Hong, Hyunjin Jung, YoungJu Jo, Byung-Ha Oh, Byung Ouk Park, Won Do Heo

Issue&Volume: 2019-10-14

Abstract: 

Intracellular antibodies have become powerful tools for imaging, modulating and neutralizing endogenous target proteins. Here, we describe an optogenetically activated intracellular antibody (optobody) consisting of split antibody fragments and blue-light inducible heterodimerization domains. We expanded this optobody platform by generating several optobodies from previously developed intracellular antibodies, and demonstrated that photoactivation of gelsolin and β2-adrenergic receptor (β2AR) optobodies suppressed endogenous gelsolin activity and β2AR signaling, respectively.

DOI: 10.1038/s41592-019-0592-7

Source: https://www.nature.com/articles/s41592-019-0592-7